The demonstration was held near the Tyendinaga Mohawk Territory in Marysville, Ont., between Belleville and Kingston.
VIA Rail had stopped train service along the Toronto–Ottawa and Toronto–Montreal corridors because of the protest.
The OPP made several arrests and charged three men, including Tyendinaga spokesperson Shawn Brant, according to The Canadian Press.
The rally, which was held beside the tracks, began at 9:30 a.m. Tuesday and comes after the February murder of an Inuk woman, Loretta Saunders, in Nova Scotia.
— Windspeaker (@windspeakernews) March 19, 2014
It ended Wednesday evening.
Napanee – blockade on CN tracks at Wyman Rd has ended peacefully. Protesters have left, Wyman Rd reopened . Police continue to investigate.
— OPP East (@OPP_ER) March 19, 2014
Three aboriginal women died during 2013 in Toronto, all of them under disputed circumstances.
VIA Rail was busing commuters along the busy corridors, causing frustration among those travelling on Tuesday and Wednesday.